Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-3 is synthesized as an inactive pro-enzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases,as well as relevant targets in the cells (e.g.,PARP and DFF).
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects proform and the cleaved large fragment of caspase-3.
免疫原
Synthetic peptide corresponding to the C-terminus of caspase-3 large fragment
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Caspase-3蛋白发生免疫反应.
保存建议
提供的Caspase-3抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Caspase-3抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.