Caspase-7 is a 34 kDa protein consisting of two subunits and a short prodomain. The procaspase-7 can be activated by cleavage by upstream caspases to form active caspase-7 in apoptosis. Once activated,caspase-7 cleaves many of the same substrates as caspase-3,including poly (ADP-ribose) polymerase (PARP) and sterol regulatory element binding proteins (SREBPs).
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects the full length (35-37 kDa) and the large fragment (20-25 kDa) of processed caspase-7 by Western blotting. Mouse small intestine tissue lysate can be used as a positive control.
免疫原
Synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-7 large fragment
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Caspase-7蛋白发生免疫反应.
保存建议
提供的Caspase-7抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Caspase-7抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.