Tumor necrosis factor receptor 1 and 2 (TNF-R1 and TNF-R2) are 55 and 75 kDa. While TNF-R1 and TNF-R2 share 28% sequence homology in the extracellular domains,their intracellular domains lack sequence homology,suggesting that they differ in their internal signal transduction pathways. TNF-R1 contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF-R1 associated death domain protein),and subsequent interactions with FADD. TNF-R1 can also activate the transcription factor NFkB via TRAF2 (TNF receptor associated factor 2). The cytoplasmic domain of TNF-R1 can directly interact with Jak kinase,thereby activating the JAK/STAT signal transduction cascade.
应用类型
Western blotting (0.5-4 ug/ml) and in Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects cdc42 of human, mouse, rat, and bovine origins.
免疫原
Synthetic peptide surrounding amino acid 32 of human TNF-RI
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、仓鼠、牛、兔、猴种属来源的TNF-R1蛋白发生免疫反应.
保存建议
提供的TNF-R1抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该TNF-R1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.