Members of the Bcl-2 family proteins all contain Bcl-2 homology domains including BH1,BH2,BH3,and BH4. The BH3 domain is known to be important for the cell killing activity of Bcl-2 members including Bad,Bax,Bid,Bik,and the recently identified BH3 domain containing protein designated Bim/BOD. Bim/BOD interacts with diverse members of the Bcl-2 sub-family including Bcl-2,Bcl-x,and Bcl-w,to induce apoptosis. There are three splice variants: BimL,BimS,and BimEL,all of which share homology in their C-terminal BH3 domains. Of the isoforms,BimS is the most potent inducer of apoptosis. The mRNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 20 kDa Bim/BOD in samples from human, mouse and rat origins. Jurkat cell lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 25 of human Bim