Wee1 is a nuclear tyrosine protein kinase. The protein plays a role in suppressing eukaryotes’ entry into mitosis by inactivating cdc2 kinase by inhibiting tyrosine phosphorylation. The predicted molecular weight of the kinase is 72 kDa. The kinase possesses tyrosine kinase activity,but structurally belongs to the serine/threonine protein kinase family.
应用类型
Western blot analysis (0.5-4 ug/ml) and in in immunoprecipitation (5-10 ug/ml). However, the optimal conditions should be determined individually. The affinity-purified rabbit antibody recognizes Wee1 protein from human, mouse and rat samples. Mouse small intestine tissue lysate (Cat.# 2601-100) can be used as a positive control
免疫原
Synthetic peptide mapping to the C-terminus of human Wee1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Wee1蛋白发生免疫反应.
保存建议
提供的Wee1抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Wee1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.