Human Raf1 is a proto-oncogene-encoded 74 kDa kinase that serves as an upstream activator of Mek1 by phosphorylating Ser-218 and Ser-222 in the MAP kinase kinase （Mek1）. It has also been implicated in the phosphorylation and activation of the cdc2 tyrosine phosphatase cdc25. Raf1 binds to the GTP-bound form of the Ras G-protein via an N-terminal domain in Raf-1. Raf-1 is activated upon phosphorylation by protein kinase C，Src and ceramide-activated protein kinase. Raf-1 is highly related to the A-Raf and B-Raf isoforms within the N-terminal G-protein binding domain （CR1），phosphorylation site domain （CR2） and the catalytic domain （CR3）. Raf-1 physically interacts with Ras，RapIB，MEK1，the 14-3-3 family of proteins and the molecular chaperones hsp90 and p50.
应用类型
Western blot analysis （1-4 ug/ml）， immunoprecipitation （15-25 ug/ml） and Immunohistochemistry （10-20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects a 74 kDa Raf-1 protein on SDS-PAGE immunoblots. It is non cross-reactive with Raf-A and Raf-B.
免疫原
Synthetic peptide mapping to the C-terminus of human Raf-1
来源宿主
兔
反应性
经检测验证，可与人、小鼠、大鼠种属来源的Raf1蛋白发生免疫反应.
保存建议
提供的Raf1抗体产品为100 ug 无色溶液，其浓度为0.2 mg/ml，溶解于包含有50%甘油， 1% BSA， 0.02%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Raf1抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.
