Protein kinase A (PKA) also known as cAMP-dependent protein kinase,is a 40 kDa protein kinase that phosphorylates serine or threonine residues in target proteins in response to elevated levels of cAMP. It has been suggested that the complex structure of PKA may be necessary for it to accomplish such diverse functions.
应用类型
Western blot analysis (0.5-4 ug/ml) and in immunoprecipitation (20-30 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide surrounding amino acid 11 of the catalytic subunit of human PKA alpha isoform
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、牛种属来源的PKA蛋白发生免疫反应.
保存建议
提供的PKA抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该PKA抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.