AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5’-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1,a member of the Ser/Thr protein kinase family,is a catalytic subunit of AMPK.
应用类型
The antibody can be used for ELISA (0.25 ug/ml) and Western blotting (0.5 – 2.5 ug/ml).
免疫原
Synthetic peptide selected from the C-terminal region of human AMPK alpha 1
来源宿主
兔
反应性
经检测验证,可与人、小鼠种属来源的AMPK⍺1蛋白发生免疫反应.
保存建议
提供的AMPK⍺1抗体产品为100 ug 无色溶液,其浓度为0.25 mg/ml,溶解于包含有0.09% (W/V) 叠氮钠的PBS缓冲液中.请将该AMPK⍺1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.