MAP kinase-activated protein kinase-2 (MAPKAPK-2),also known as p45 hsp27 kinase,is a 45-54 kDa serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites. MAPKAPK-2 is activated in response to stress,IL-1 and TNF,possibly catalyzed by p38/Hog-dependent phosphorylation. One of the major substrates of MAPKAPK-2 is hsp27,which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. Two isoforms are produced due to alternative splicing of the same gene which differs in their C-terminals. This antibody recognizes both isoforms.
应用类型
Western blot analysis (0.5-4 ug/ml) and immunoprecipitation. However, the optimal conditions should be determined individually. The affinity purified antibody detects both 43 and 60 kDa isoforms, corresponding to the apparent molecular mass of MAPKAPK-2 on SDS-PAGE immunoblots. These 43 kDa and 60 kDa bands can be specifically inhibited by the relevant peptide.
免疫原
Synthetic peptide surrounding amino acid 365 of mouse MAPKAPK-2
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、狗、牛种属来源的MAPKAPK-2蛋白发生免疫反应.
保存建议
提供的MAPKAPK-2抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该MAPKAPK-2抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.