Hsp90 (also known as Hsp82, Hsp83, or Hsp89), a 90 kDa protein, is the most abundant protein in the cytosolic fraction in many cell types. The high level of Hsp90 in many cell types suggests that Hsp90 may play a general role in the cell, but little is known about its general function. Hsp90 has no known enzymatic activity, and thus it has been presumed that Hsp90 may function through protein-protein interactions. Hsp90 exists in a dimeric form and has been observed to bind to several cellular proteins such as retro-virus kinases (PP60v-src), steroid receptors, heme-regulated protein kinase, actin and tubulin. In this regard, Hsp90, like Hsp70, may function as a “molecular chaperone”. Hsp90 exists as two isoforms referred to as Hsp86 and HSP84 in murine cells or Hsp90α and Hsp90β in human cells.
应用类型
Western blot analysis (1-2ug/ml), immunoprecipitation (4-8 ug/ml) and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes a 90 kDa protein, corresponding to the apparent molecular weight of Hsp90 on SDS-PAGE immunoblots. This antibody detects both Hsp90 alpha and beta.
免疫原
Achlya ambisexualis Hsp90
来源宿主
小鼠
反应性
经检测验证,可与人、小鼠、大鼠、猴、仓鼠、兔、牛种属来源的Hsp90蛋白发生免疫反应.
保存建议
提供的Hsp90抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有1 mg/ml BSA, 50%甘油和0.2%硫柳汞的PBS缓冲液中.请将该Hsp90抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.