Hsp60,also known as Cpn60,is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell stress or shock. It is present in all species analyzed so far and exhibits a remarkable sequence homology among various counterparts in bacteria,plants,and mammals with more than half of the residues identical between bacterial and mammalian Hsp60.
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~60 kDa of Hsp60 from samples of human, mouse and rat origins. Reactivity to other species has not been determined.
免疫原
Synthetic peptide mapping to the N-terminus of human Hsp60