Human Hsp27,mouse Hsp25 and B-crystallin are part of a diverse family of small heat shock proteins which are produced in all organisms. They function as chaperone-like proteins by binding unfolded polypeptides and preventing uncontrolled protein aggregation. Hsp27 is believed to exist mainly as oligomers of as many as 8-40 Hsp27 protein monomers in cells and data suggests that the large oligomers of Hsp27 have a chaperone-like activity by serving as a site where unfolding proteins may bind until ATP and Hsp70-dependent refolding can occur. Hsp27 is believed to protect cells by enhancing cellular glutathione levels and elevated glutathione levels have been measured in cells overexpressing Hsp27. Data from studies using wild-type Hsp27 and mutant forms in which the serine phosphorylation sites were mutated to alanines,glycines or aspartates,have shown that cellular glutathione levels depend on the oligomerization of Hsp27. Recent findings indicate that Hsp27 is also a negative regulator of cytochrome c-dependent activation of procaspase-3.
应用类型
Western blotting (1-2 ug/ml), immunoprecipitation (5-10 ug/ml), immunocytochemistry (10-15 ug/ml), and ELISA (1 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 27 kDa protein, corresponding to the apparent molecular mass of Hsp27 on SDS-PAGE immunoblots, in samples from human, monkey, dog (weakly) and pig (weakly) origins.
免疫原
Recombinant human Hsp27
来源宿主
兔
反应性
经检测验证,可与人/猴种属来源的Hsp27蛋白发生免疫反应.
保存建议
提供的Hsp27抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Hsp27抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.