cdc42/Rac belongs to the superfamily of small GTPases that are structurally linked to the proto-oncogene product p21ras and are important for the control of cell growth and differentiation as well as for intracellular organization. Cdc42/Rac is an important upstream regulator of the protein kinase cascade that controls the SAPK/JNK and p38 activity. Recent data also suggest that constitutive active forms of cdc42 can induce apoptosis through a mechanism requiring signaling through SAPK/JNK.
应用类型
Western blotting (0.5-4 ug/ml) and in Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually. The antibody detects cdc42 of human, mouse, rat, and bovine origins.
免疫原
Synthetic peptides surrounding amino acid 144 of human cdc42
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、牛种属来源的cdc42/Rac蛋白发生免疫反应.
保存建议
提供的cdc42/Rac抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该cdc42/Rac抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.