Calreticulin is a calcium binding protein found in abundance in the endoplasmic reticulum and the sarcoplasmic reticulum. Like many other ER proteins,it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus. Calreticulin has also been detected in the nucleus and nuclear envelop. Recent studies suggest that this soluble ER protein has a multifunctional role as it appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. Studies also suggest its involvement in certain autoimmune diseases. Consistence with its multiple functions the calreticulin molecule appears to have a zonal character. The protein has both high and low affinity calcium binding sites.
应用类型
Western blot analysis (0.5-4 ug/ml) and in Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide mapping to the C-terminus of Human Calreticulin
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Calreticulin蛋白发生免疫反应.
保存建议
提供的Calreticulin抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5 mg/ml BSA和0.01%硫柳汞的PBS缓冲液中.请将该Calreticulin抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.