Granzyme B is a 27 kDa serine protease stored in granules of activated cytotoxic T cells and NK cells. Upon target cell contact,granzyme B is directionally exocytosed and assisted by perforin,enters the target cell. With its unique substrate specificity (granzyme B cleaves after Asp),granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 221 of human Granzyme B
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Granzyme B 蛋白 发生免疫反应.
保存建议
提供的Granzyme B 抗体 产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Granzyme B 抗体 产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.