CD40 is a type I membrane protein found on the surface of B cells and primary carcinomas. It belongs to the TNF-R family. CD40 serves as the receptor for CD40 ligand (CD40L),a cytokine expressed on the cell surface of T cells. CD40 plays a critical role in B cell proliferation,antibody class switching,modulation of apoptosis in the germinal center through interaction with T cells expressing CD40L,and activation of CD4+ T cells. In non-hemopoitic cells such as epidermal basal cells,CD40 ligation serves as the signal for inhibition of cell growth and induction of differentiation. The cytoplasmic domain of CD40 interacts with TRAF6,a TNFR associated factor homolog that mediates signaling from CD40. Ligation of CD40 with its ligand preferentially induces stress-activated protein kinases in B lymphocytes.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 261 of human CD40
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、猴、猪种属来源的CD40蛋白发生免疫反应.
保存建议
提供的CD40抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该CD40抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.