Apoptosis can be induced by certain cytokines including TNF and Fas Ligand of the TNF super-family through their death domain (DD) containing receptors,TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR4 (death receptor 4) and TRAIL-R1. The ligand for this novel death receptor has been identified and termed TRAIL. DR4 is expressed in most of the human tissues including spleen,peripheral blood leukocytes (PBLs),small intestine and thymus. Like TNFR1,Fas and DR3,DR4 mediates apoptosis and NFkB activation in many tissues and cells.
应用类型
Western blot analysis (0.5-4 ug/ml) and immunofluorescence. However, the optimal conditions should be determined individually. The antibody detects a 57 kDa mouse DR4 in Western blot analysis.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 454 of human DR4
来源宿主
兔
反应性
经检测验证,可与小鼠种属来源的DR4蛋白发生免疫反应.
保存建议
提供的DR4抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该DR4抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.