c-FLIP was cloned by several labs independently and termed Casper,I-FLICE,FLAME-1,CASH AND CLARP. c-FLIP contains two death effector domains and has sequence homology to caspase-8. c-FLIP interacts with adapter protein FADD and caspaser-8 and -10,and potently inhibits apoptosis induced by all known death receptors. Two splice variants of c-FLIP have been identified and termed FLIPl and FLIPs
应用类型
Western blot analysis (0.5-4 ug/ml) and immunofluorescence. However, the optimal conditions should be determined individually. The immunoaffinity-purified antibody detects 58 kDa FLIPL on SDS-PAGE immunoblots, in samples from human, mouse and rat origins. The antibody recognizes only the FLIPL form.
免疫原
Synthetic peptide near the C-terminus of human FLIPa/FLIPL form
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的c-FLIP蛋白发生免疫反应.
保存建议
提供的c-FLIP抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该c-FLIP抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.