Apoptosis can be induced by certain cytokines including TNF and Fas in the TNF superfamily through interaction with their death domain (DD) containing receptors,TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR3 (also called Wal-1,Apo3,TRAMP and LARD). The ligand for this novel death receptor has been defined as TWEAK (also termed Apo3L). Like TNFR1,overexpression of DR3 triggers apoptosis and NF-kB activation. Activation of DR3 results in recruitment of TRADD (a DD containing adapter molecule). TRADD associates with FADD,TRAF2,and RIP to activate the signaling complex for NF-kB activation. DR3 activates apoptosis through TRADD,FADD,and caspase-8. DR3 is highly expressed in the tissues enriched in lymphocytes including peripheral blood leucocytes (PBL),thymus and spleen.
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to residues surrounding amino acids 378 of mouse DR3