During apoptosis,various stimuli activate a protease cascade of caspases that eventually cause degradation of chromosomal DNA. DFF45 (DNA fragmentation factor 45) is a down-stream regulator of DNA degradation (DFF40). DFF40 is an apoptotic DNase with a high specific activity that exists in an inactive complex with DFF45 in living cells. Cleavage of DFF45,which is mediated by caspase-3,leads to DFF40 release where it then enters nucleus to function as a nuclease. DFF45 may inhibit the DNAse activity of DFF40 and its nuclear translocation. Activation of DFF40 causes DNA degradation,a hallmark of apoptosis.
应用类型
Western blot analysis (0.5-4 ug/ml). However, the optimal dilutions should be determined individually. Recognizes 45 and 40 KDa proteins, corresponding to the apparent molecular weight of DFF45/ICADL and DFF45/ICADs respectively.
免疫原
Recombinant human DFF45-GST fusion protein
来源宿主
小鼠
反应性
经检测验证,可与人种属来源的DFF45蛋白发生免疫反应.
保存建议
提供的DFF45抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 0.5% BSA和0.02%硫柳汞的PBS缓冲液中.请将该DFF45抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.