DFF is composed of two subunits: DFF40/CAD and DFF45/ICAD. Human DFF45 and its mouse homologue ICAD are the inhibitors of DFF40 and CAD,respectively. Cleavage of DFF45,which is mediated by caspase-3,leads to DFF40’s activation as a nuclease. Activation of DFF40/CAD can lead to DNA fragmentation,a hallmark of apoptosis. DFF40 mRNA is expressed in limited number of human tissues: pancreas,spleen,prostate,and ovary.
应用类型
Western blotting (0.5-4 ug/ml) and Immunohistochemistry (2-8 ug/ml). However, the optimal conditions should be determined individually. The antibody detects a 43 kDa DFF40/CAD of human, mouse and rat origins. Mouse kidney tissue lysate can be used as a positive control.
免疫原
Synthetic peptide surrounding amino acid 208 of mouse DFF40
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的DFF40蛋白发生免疫反应.
保存建议
提供的DFF40抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该DFF40抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.