Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer as well as many other types of diseases. Recently,a human DNA fragmentation factor (DFF45) was identified. DFF is composed of two subunts: DFF40 and DFF45. Cleavage of DFF45,which is mediated by caspase-3,leads to DFF40’s activation as a nuclease. Activation of DFF40 can lead to DNA fragmentation,a hallmark of apoptosis.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The antibody detects the 45 kDa and 28 kDa proteins, corresponding to the apparent molecular weight of DFF45/ICADL, and ICADS in immunoblots.
免疫原
Synthetic peptide mapping to the N-terminus of mouse DFF45
来源宿主
兔
反应性
经检测验证,可与小鼠、大鼠种属来源的DFF45蛋白发生免疫反应.
保存建议
提供的DFF45抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该DFF45抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.