Bok (Bcl-2-related ovarian killer) is a pro-apoptotic Bcl-2 family protein identified in the ovary based on its dimerization with the anti-apoptotic protein Mcl-1. In addition to the Bcl-2 homology (BH) domains 1 and 2 and the transmembrane sequence,Bok also has a BH3 domain believed to be important for dimerization with selective anti-apoptotic Bcl-2 proteins and cell killing. Bok interacts strongly with some (Mcl-1,BHRF1,and Bfl-1) but not other (Bcl-2,Bcl-xL,and Bcl-w) anti-apoptotic members. In addition,cell killing induced by Bok was suppressed following coexpression with Mcl-1 and BHRF1,but not with Bcl-2,further indicating that Bok heterodimerized only with selective anti-apoptotic Bcl-2 proteins. Bok was highly expressed in the ovary,testis and uterus.
应用类型
Western blot analysis (0.5-4 ug/ml).
免疫原
Synthetic peptide surrounding amino acid 44 of rat BOK
来源宿主
兔
反应性
经检测验证,可与小鼠、大鼠种属来源的Bok蛋白发生免疫反应.
保存建议
提供的Bok抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Bok抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.