Fas-associating death domain containing protein (FADD),also known as mediator of receptor induced toxicity (MORT1),is a 23 kDa cytosolic protein with an apparent molecular weight of 28 kDa on SDS-PAGE. The protein is involved in both Fas and TNF-R induced cell death pathway. FADD specifically binds to Fas to initiate the apoptotic signalling cascade. Overexpression of FADD induces apoptosis.
应用类型
Western blot analysis (0.5-4 ug/ml)and can also be used in immunoprecipitation. However, the optimal conditions should be determined individually. The antibody detects an ~28 kDa band, corresponding to the expected molecular mass of FADD on immunoblots.
免疫原
Synthetic peptide surrounding amino acid 128 of human FADD
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、猴种属来源的FADD蛋白发生免疫反应.
保存建议
提供的FADD抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该FADD抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.