Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1,or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-B1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells,Mcl-1 interacts with another Bcl-2-related protein,Bax,and prolongs cell viability after treatment with different apoptotic reagents.
应用类型
Western blot analysis (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal conditions should be determined individually.
免疫原
Synthetic peptide corresponding to residues surrounding amino acid 131 of human Mcl-1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、猴种属来源的Mcl-1蛋白发生免疫反应.
保存建议
提供的Mcl-1抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Mcl-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.