The Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bak) apoptosis. Bax contains the conserved Bcl-2 homology 1 (BH1) and 2 (BH2) domains that allow for its homodimerization or heterodimerization with Bcl-2. When Bcl-2 is in excess,apoptosis is inhibited. However,if Bax levels increase in response to a death signal,the cell is pushed toward death.
应用类型
Western blot analysis (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually.The antibody detects a 20 kDa protein, corresponding to the apparent molecular weight of Bax on SDS-PAGE immunoblots.
免疫原
Synthetic peptide corresponding to amino acids near the N-terminus of human Bax
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、猴种属来源的Bax蛋白发生免疫反应.
保存建议
提供的Bax抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Bax抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.