The Bcl-2 (B-cell leukemia/lymphoma 2) family proteins function to either inhibit (Bcl-2 and Bcl-XL) or promote (Bax and Bad) apoptosis. Similar to other family member,Bad also exhibits sequence homology to Bcl-2. Bad can selectively dimerize with Bcl-xL and Bcl-2,but not with Bax,Bcl-xs,Mcl-1,or itself. In mammalian cells,Bad binds more strongly to Bcl-xL than Bcl-2. This may explain why Bad reverse the death repressor activity of Bcl-xL,but not that of Bcl-2
应用类型
Western blot analysis (1-2 ug/ml), immunoprecipitation (4-8 ug/ml), and Immunohistochemistry (20-40 ug/ml). However, the optimal conditions should be determined individually. The antibody detects the 22 kDa Bad protein and it does not cross-react with related family members.
免疫原
Synthetic peptide surrounding amino acid 115 of mouse Bad
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、猴种属来源的Bad蛋白发生免疫反应.
保存建议
提供的Bad抗体产品为100 ug 无色溶液,其浓度为0.5 mg/ml,溶解于包含有50%甘油, 1% BSA, 0.02%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Bad抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.