Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-5 is also known as Ich-3 and ICErel-III and can proteolytically processed by granzyme B. Over expression of caspase-5 induces apoptosis in Rat-1 and HeLa cells,which can be inhibited by CrmA and Bcl-2.
应用类型
Western blotting (0.5-4ag/ml). However, the optimal conditions should be determined individually. The affinity purified antibody preferentially detects the intermediate (32 kDa) and the fully (20 kDa) cleaved caspase-5.
免疫原
Synthetic peptide surrounding amino acids 162 of human caspase-5
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、猴、仓鼠、牛种属来源的Caspase-5蛋白发生免疫反应.
保存建议
提供的Caspase-5抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA和0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Caspase-5抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.