Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.
应用类型
Western blotting (0.5-4 ug/ml). The immunoaffinity purified antibody detects the full length (55 kDa), intermediate cleavage fragments (26-31 kDa) and cleaved large fragment (18 kDa) of caspase-8.
免疫原
Synthetic peptide surrounding amino acid 392 of mouse caspase-8
来源宿主
兔
反应性
经检测验证,可与小鼠、大鼠、牛、猴种属来源的Caspase-8蛋白发生免疫反应.
保存建议
提供的Caspase-8抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.5% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Caspase-8抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.