During apoptosis,Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2),a reaction that requires dATP,and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9,and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3,one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues.
应用类型
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots.
免疫原
Synthetic peptide surrounding amino acid 1161 of human Apaf-1
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠、牛、兔、猴、马种属来源的Apaf-1蛋白发生免疫反应.
保存建议
提供的Apaf-1抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有30%甘油, 0.05% BSA, 0.01%硫柳汞的PBS (pH 7.2)缓冲液中.请将该Apaf-1抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.