Caspase-3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase-3 proteolytically cleaves and activates other caspases,as well as relevant targets in the cells (e.g.,PARP). The affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
应用类型
Western blotting (1:50 -1:500 dilutions) and Immunohistochemistry (1:10-1:20 dilutions). However, the optimal concentrations should be determined individually. The antibody preferentially recognizes the p17 fragment of the active caspase-3 in samples from human, mouse, and rat origins
免疫原
Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
来源宿主
兔
反应性
经检测验证,可与人、小鼠、大鼠种属来源的Caspase-3 (Active)蛋白发生免疫反应.
保存建议
提供的Caspase-3 (Active)抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有50%甘油, 1% BSA和0.02%硫柳汞的PBS缓冲液中.请将该Caspase-3 (Active)抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.