NFkB was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor,IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS),pro-inflammatory cytokines,and viral infection. IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
应用类型
Western blot analysis (1-4 ug/ml). However, the optimal conditions should be determined individually. A431 cells can be used as a positive control.
免疫原
Synthetic peptide from human NFkB
来源宿主
小鼠
反应性
经检测验证,可与人、小鼠、大鼠种属来源的NFkB p65蛋白发生免疫反应.
保存建议
提供的NFkB p65抗体产品为100 ug 无色溶液,其浓度为0.2 mg/ml,溶解于包含有1 mg/ml BSA和1.5 mM 叠氮钠和50%甘油.的PBS缓冲液中.请将该NFkB p65抗体产品置于-20 ℃冷冻保存,建议分装使用以避免反复冻融.