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细胞分析试剂盒
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中文名称

DNA Isolation Kit, Saliva, BioAssay

英文名字
DNA Isolation Kit, Saliva, BioAssay
供应商
USBiological
产品货号
299009
产品报价
¥1/50Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Saliva or septum samples, the isolation procedure was designed for purification from 200ul of sample but may be used to isolate DNA from up to 600ul of saliva sample. If increasing the sample volume from 200ul, adjust the amount of Lysis Binding Buffer C, Proteinase K Solution, and Isopropanol proportionately.Intended Use:The Saliva DNA Isolation BioAssay Kit is designed for the purification of genomic DNA and circulating DNA from freshly collected saliva samples. Range:50-200ul of sampleDNA Yield: 0.25-4ug/200ul fresh salivaTest Principle:Purification is performed with the use of Spin columns which bind the DNA. No phenol-chloroform and precipitation steps are involved in this procedure. Isolation of the DNA is performed by lysing the cells in a solution containing chaotropic salts and detergent. To assist inlysing the cells, proteinase K is also added to the mixture. Isopropanol is added to the samples, before applying to the spin columns. Under these conditions the DNA binds to the membrane while other contaminants are washed through. The columns are then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final genomic DNA product is eluted in TE. The final DNA product can be used directly for quantitative PCR and other downstream applications.Kit Components:Lysis Binding Buffer C, 1x30ml, RTWash Buffer 1, 1x14ml, RTWash Buffer 2, 1x7ml, RTTE Buffer, 1x10ml, RTSpin column set, 50 units, RTProteinase K Solution, 1x250ul, -20°C* Wash buffers 1 and 2 are provided as concentrates that require the addition of 100% ethanol to them before use.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对DNA Isolation Kit, Saliva, BioAssay 该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到DNA Isolation Kit, Saliva, BioAssay 产品后将其置于RT/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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