- 中文名称
SETD2, Chemiluminescent, BioAssay Kit (SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732)
- 英文名字
- SETD2, Chemiluminescent, BioAssay Kit (SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732)
- 供应商
- USBiological
- 产品货号
- 298456
- 产品报价
- ¥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
- 产品描述
- SETD2 is a histone methyltransferase that exhibits methylation activity toward histones H3-K36.Intended Use:The SETD2 Direct Activity Chemiluminescent BioAssay Kit is designed to measure SETD2 activity for screening and profiling applications. Test Principle:The key to the SETD2 Chemiluminescent BioAssay Kit is a highly specific antibody that recognizes methylated K36 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Contraindications:Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.Applications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.Kit Components:Plate precoated with histone substrate, 1x96 wellsSETD2 human enzyme, 1x40ug S-adenosylmethionine, 1x250ul Primary antibody 12, 1x100ul Secondary HRP-labeled antibody 2, 1x10ul 4x HMT assay buffer 1, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent substrate, 2 components x 6ml each Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对SETD2, Chemiluminescent, BioAssay Kit (SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732)该产品的特点优势,欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到SETD2, Chemiluminescent, BioAssay Kit (SET Domain Containing 2, HYPB1, HIF-1, KMT3A, KIAA1732)产品后将其置于4°C/-20°C/-70°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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