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细胞分析试剂盒
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中文名称

PRMT3, Chemiluminescent, BioAssay Kit (Protein Arginine Methyltransferase 3, HRMT1L3)

英文名字
PRMT3, Chemiluminescent, BioAssay Kit (Protein Arginine Methyltransferase 3, HRMT1L3)
供应商
USBiological
产品货号
298450
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
PRMT3 is a histone methyltransferase that exhibits methylation activity toward H4-R3.Intended Use:The PRMT3 Direct Activity Chemiluminescent BioAssay kit is designed to measure PRMT3 activity for screening and profiling applications. Test Principle:The key to the PRMT3 Direct Activity BioAssay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.Contraindications:DMSO >1%, strong acids or bases, ionic detergents, high saltApplications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.Kit Components:Plate precoated with histone substrate, 1x96 wellsPRMT3 human recombinant enzyme, 1x10ug S-adenosylmethionine*, 20um, 1x250ul Primary antibody 4, 1x100ul Secondary HRP-labeled antibody 2, 1x10ul HMT assay buffer 2, 4x, 1x3ml Blocking buffer, 1x50ml HRP chemiluminescent A, (transparent bottle), 1x6ml HRP chemiluminescent B, (brown bottle), 1x6ml *Decreasing S-adenosylmethionine concentration will make the assay more sensitive to theinhibitors.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对PRMT3, Chemiluminescent, BioAssay Kit (Protein Arginine Methyltransferase 3, HRMT1L3)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到PRMT3, Chemiluminescent, BioAssay Kit (Protein Arginine Methyltransferase 3, HRMT1L3)产品后将其置于4°C/-20°C/-70°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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