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细胞分析试剂盒
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中文名称

PARP2, Chemiluminescent, BioAssay Kit (Poly Polymerase 2, Poly Transferase 2, ADP-Ribose, ADPRT)

英文名字
PARP2, Chemiluminescent, BioAssay Kit (Poly Polymerase 2, Poly Transferase 2, ADP-Ribose, ADPRT)
供应商
USBiological
产品货号
298439
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
PARP2 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.Intended Use:The PARP2 Chemiluminescent Activity BioAssay kit is designed to measure PARP2 activity for screening and profiling applications. Test Principle:The key to the PARP2 Chemiluminescent Activity BioAssay is the biotinylated substrate. With this kit, only three simple steps are required for PARP2 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP2 biotinylated substrate is incubated with the assay buffer that contains the PARP2 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader. This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected.Contraindications:DMSO >1%, strong acids or bases, ionic detergents, high saltApplications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.Kit Components:Module plate, 1x96 wellsPARP2, 1x5ug Histone mixture, 5x, 1x1ml Assay Mixture Containing Biotinylated Substrate, 10x, 1x300ulPARP assay buffer, 10x, 1x1ml Blocking buffer, 1x25ml Activated DNA (5x), 1x500ul Streptavidin-HRP, 1x100ul HRP chemiluminescent substrate A, (translucent bottle), 1x6ml HRP chemiluminescent substrate B, (brown bottle), 1x6ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对PARP2, Chemiluminescent, BioAssay Kit (Poly Polymerase 2, Poly Transferase 2, ADP-Ribose, ADPRT)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到PARP2, Chemiluminescent, BioAssay Kit (Poly Polymerase 2, Poly Transferase 2, ADP-Ribose, ADPRT)产品后将其置于4°C/-20°C/-70°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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