- 中文名称
Myc Reporter, BioAssay Kit (Myc Signaling Pathway)
- 英文名字
- Myc Reporter, BioAssay Kit (Myc Signaling Pathway)
- 供应商
- USBiological
- 产品货号
- 298433
- 产品报价
- ￥1/500Tests
- 产品说明书
- 点击查看
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The Myc signaling pathway plays an important role in cell proliferation, differentiation, transformation and apoptosis. The c-Myc protein is a transcription factor that heterodimerizes with Max to regulate Myc signaling pathway responsive genes. Myc mutations have been linked to the development of a number of human cancers, including Burkitt’s lymphoma, cervical, ovarian, breast, lung and pancreatic carcinoma, making Myc a promising therapeutic target for cancer treatment.Sample Type:Cultured cellsIntended Use:To monitor Myc signaling pathway activity, screen activators or inhibitors of the Myc signaling pathway and to study effects of RNAi or gene overexpression on the activity of the Myc pathway.Test Principle:The Myc Pathway Reporter BioAssay kit is designed for monitoring the activity of the Myc signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for c- Myc and Myc luciferase reporter vector. Inside the cells, c-Myc will bind to Max, translocate to the nucleus, and induce expression of the Myc luciferase reporter vector. This reporter contains the firefly luciferase gene under the control of multimerized Myc responsive elements located upstream of a minimal promoter. The Myc reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency.The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity.Kit Components:Reporter (Component A), Myc luciferase reporter vector + constitutively-expressing Renillaluciferase vector, 1x500ul, (60ng DNA/ul)Negative Control Reporter (Component B), Non-inducible luciferase vector + constitutively-expressing Renilla luciferase vector, 1x500ul, (60ng DNA/ul)c-Myc Expression Vector (Component C), c-Myc expression vector, 1x500ul, (100ng DNA/ul)Negative Control Expression Vector (Component D), Empty expression vector without c-Myc, 1x500ul (100ng DNA/ul)The vectors are suitable for transient transfection. They are NOT suitable for transformation and amplification in bacteria.Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Myc Reporter, BioAssay Kit (Myc Signaling Pathway)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Myc Reporter, BioAssay Kit (Myc Signaling Pathway)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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