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细胞分析试剂盒
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中文名称

LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)

英文名字
LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)
供应商
USBiological
产品货号
298427
产品报价
¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
LSD1 is a chromatin-modifying enzyme that specifically removes methyl groups from mono- and dimethylated Lys of histone H3. LSD1 is a critical component of transcriptional regulation via epigenetic histone modifications and is therefore a potential target for drug development.Intended Use:The LSD1 BioAssay Kit is designed to measure LSD1 activity of purified LSD1 enzymes containing LSD1 using fluorimetry. The assay is based on the analysis of the hydrogen peroxide produced during the demethylation reaction mediated by LSD1. Test Principle:The key to the LSD1 BioAssay Kit is the methylated peptide substrate. Using this kit, only two simple steps on a microtiter plate are required for LSD1 reactions. First, a sample containing LSD1 is incubated in a reaction mixture with the methylated peptide substrate. Second, peroxidase and Amplex Red reagent are added and fluorescence (lamda ex=530±13 nm, lambda em=590±18 nm) is measured using a plate reader.Contraindications:DMSO >1%, strong acids or bases, ionic detergents, high saltApplications:Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications.Kit Components:White microtiter plate, 1x 96 wellsLSD1 recombinant enzyme, 1x30ug Methylated peptide substrate (200uM) 1x500ul Amplex Red reagent (10mM) 1x50ulPeroxidase (10U/ml), 1x100ul 2x LSD1 assay buffer, 1x20ml Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
产品特点
针对LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到LSD1, Fluorescent, BioAssay Kit (Lysine-Specific Histone Demethylase, KDM1A, AOF2, BHC110)产品后将其置于4°C/-20°C/-70°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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