- 中文名称
Periostin, BioAssay ELISA Kit (Postn, Osteoblast-specific Factor 2, OSF-2) (Human)
- 英文名字
- Periostin, BioAssay ELISA Kit (Postn, Osteoblast-specific Factor 2, OSF-2) (Human)
- 供应商
- USBiological
- 产品货号
- 207334
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Periostin was originally isolated as an osteoblast-specific factor that functions as a cell adhesion molecule for pre-osteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta1 (TGF-beta1) and bone morphogenetic protein 2 (BMP-2). Abnormal expression of periostin is also linked to angiogenesis and metastasis in epithelial tumors. Periostin up-regulation in cancers usually correlates with aggressiveness and/or poor survival.Sample Type:Cell culture supernatants, serum and plasmaIntended Use:The human Periostin BioAssay ELISA Kit is to be used for the in vitro quantitative determination of human periostin in cell culture supernatants, serum and plasma. Sensitivity:15pg/mlRange:78-5000pg/mlSpecificity:Recognizes natural and recombinant human periostin. Has been tested on human periostin isoforms 1 and 2 (should also detect human isoforms 3 and 4).Test Principle:This BioAssay is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of periostin in the samples.Kit Components:Human periostin Standard, lyophilized, 1x1ugDetection Antibody, 1x20ulHRP Labeled Streptavidin, lyophilized, 1x2ugWash Buffer 10X, 2x30mlELISA Buffer 10X, 2x30mlTMB Substrate Solution, 1x12mlStop Solution, 1x12mlPlate coated with periostin antibody, 6x16 well stripsPlate covers, 2x plastic filmSilica gel, 2x minibagsStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Periostin, BioAssay ELISA Kit (Postn, Osteoblast-specific Factor 2, OSF-2) (Human)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Periostin, BioAssay ELISA Kit (Postn, Osteoblast-specific Factor 2, OSF-2) (Human)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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