Members of the glial cell line-derived neurotropic factor (GDNF) family, including GDNF and neuturin (NTN), play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRα, which is a cell surface receptor for NTN. The cDNA encoding NTNRα from human, rat, chicken and mouse have been cloned recently. NTNRα was also termed GDNFRβ Ret ligand 2 (RETL2), or TGF-β-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. GFRα-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRα-2 and the Ret PTK from a complex to transduce NTN signal and to mediate NTN function.
应用类型
Western blot analysis (1 ug/ml) and a 52 kDa band should be detected. However, the optimal conditions should be determined individually.