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中文名称

Hepatocyte Nuclear Factor 6 Alpha (HNF6a) BioAssay ECL ELISA Kit (Human)

英文名字
Hepatocyte Nuclear Factor 6 Alpha (HNF6a) BioAssay ECL ELISA Kit (Human)
供应商
USBiological
产品货号
157727
产品报价
¥1/48Test   ¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Hepatocyte Nuclear Factor 6 Alpha (HNF6a) BioAssay ECL Kit (Human) is a quantitative, in vitro, Chemiluminescence-based sandwich immunoassay (ECL) suitable for use in Tissue homogenates and other biological fluids.Test Principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 6 Alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 6 Alpha. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Nuclear Factor 6 Alpha (HNF6a) level in the sample or standard.Detection Range:0.156-10ng/mlSensitivity:The minimum detectable dose of this kit is typically less than 0.073ng/ml.Specificity:This sandwich assay has high sensitivity and excellent specificity for detection of Hepatocyte Nuclear Factor 6 Alpha (HNF6a). No significant cross-reactivity or interference between Hepatocyte Nuclear Factor 6 Alpha (HNF6a) and analogues was observed. Stability:The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Precision:Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%Kit Components:Microtiter Plate, 1x96 wells, Pre-coated, ready to use, well strip platePlate sealer for 96 wellsStandard, 2x1 vialStandard Diluent, 1x20mlDetection Reagent A, 1x120ulDetection Reagent B, 1x120ulAssay Diluent A, 1x12mlAssay Diluent B, 1x12mlSubstrate A, 1x10mlSubstrate B, 1x2mlWash Buffer, 30X, 1x20mlMaterials Required but not Supplied:1. Luminometer capable of reading 96-well microplates with the following parameters: lag time 30.0secs; read time 1.0 sec/well.2. Precision single or multi-channel pipettes and pipette tips with disposable tips. 3. Eppendorf Tubes for diluting samples.4. Deionized or distilled water.5. Absorbent paper for blotting the microtiter plate.6. Container for Wash SolutionProtocol Summary:1. Prepare all reagents, samples and standards.2. Add 100ul standard or sample to each well. Incubate 2 hours at 37°C.3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.4. Aspirate and wash 3 times.5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.6. Aspirate and wash 5 times.7. Add 100ul Substrate Solution. Incubate 10 minutes at 37°C.8. Read RLU value immediately.Sample Collection:Serum:Use a serum separator tube and allow samples to clot for two hours at RT or overnight at 4°C before centrifugation for 20 minutes at approximately 1000×g. Assay freshly prepared serum immediately or store samples in aliquot at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Plasma:Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000×g at 2-8°C within 30 minutes of collection.Remove plasma and assay immediately or store samples in aliquots at -20°C or -80°C for later use. Avoid repeated freeze/thaw cycles.Other Biological Fluids:Centrifuge samples for 20 minutes at 1000xg. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C for later use.Avoid repeated freeze/thaw cycles.Note:Samples to be used within 5 days may be stored at 4°C, otherwise samples must be stored at -20°C ( 1 month) or -80°C ( 2 months) to avoid loss of bioactivity and contamination.Sample hemolysis will influence the result, so hemolytic specimens cannot be detected.When performing the assay, bring samples to room temperature.Storage and Stability:1. For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.2. For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.3. Expiration date is 6 months from shipment date.4. It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
产品特点
针对Hepatocyte Nuclear Factor 6 Alpha (HNF6a) BioAssay ECL ELISA Kit (Human)该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Hepatocyte Nuclear Factor 6 Alpha (HNF6a) BioAssay ECL ELISA Kit (Human)产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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