Diaphorases are flavin-containing enzymes that can transfer hydrogen from reduced di- and triphosphopyridine nucleotides, including NADH and NADPH, to various dyes, which reduces the dye. Tetrazolium salts, such as iodonitrotetrazolium (INT, Item No. 16073), are commonly used dyes, as reduction produces a highly colored formazan derivative.{26709,26176} The diaphorase from C. kluyveri is a flavin mononucleotide-containing enzyme that has both NADH and NADPH dehydrogenase activities.{26708} It has an apparent molecular weight of approximately 24 kDa.{26708} This diaphorase cannot use NADH or NADPH to reduce either oxygen or cytochrome c.{26708}
产品描述
A flavin mononucleotide-containing enzyme that has both NADH and NADPH dehydrogenase activities
