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中文名称

Advanced Glycation End Product (AGE) BioAssay ELISA Kit

英文名字
Advanced Glycation End Product (AGE) BioAssay ELISA Kit
供应商
USBiological
产品货号
023185
产品报价
¥1/48Tests   ¥1/96Tests
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
The Advanced Glycation End Product (AGE) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of AGE in serum, plasma and other biological fluids.Detection Range:98.8-8,000ng/mlSensitivity:33.6ng/mlPrecision:Intra-Assay CV: <10%Inter-Assay CV: <12%Test Principle:This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for AGE has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled AGE and unlabeled AGE (standards or samples) with the pre-coated antibody specific for AGE. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of AGE in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of AGE in the sample.Kit Components:*023185A: Microtiter Plate, 96 wells, Pre-coated, ready to use*023185B: Standard, 2x1vial 023185C: Standard Diluent, 1x20ml*023185D: Detection Reagent A, 1x120ul *023185E: Detection Reagent B, 1x120ul 023185F: Assay Diluent A, 1x12ml023185G: Assay Diluent B, 1x12ml023185H: TMB Substrate, 1x9ml023185K: Stop Solution, 1x6ml023185L: Wash Buffer, 30X, 1x20mlStorage and Stability:Store *023185A, *023185B, *023185D and *023185E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Procedure Summary:1. Prepare all reagents, samples and standards.2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.3. Aspirate and wash 3 times.4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.5. Aspirate and wash 5 times.6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.
产品特点
针对Advanced Glycation End Product (AGE) BioAssay ELISA Kit该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Advanced Glycation End Product (AGE) BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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