甲基化DNA免疫沉淀试剂盒,MeDIP统统抓下来

发布者:艾美捷科技发布时间:2018-05-25

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      【前情回顾】DNA甲基化(5-mc)极速分析试剂盒   DNA甲基化免疫共沉淀技术(MeDIP or mDIP) ,是一个大范围的染色体或基因组纯化技术,在分子生物学中被用于富集DNA甲基化序列,它采用抗体或甲基化DNA结合蛋白来捕获富集甲基化DNA的技术,这种技术可以发现基因组中高度甲基化的区域,如CpG岛。MeDIP技术特别适用于高通量测序以及芯片杂交检测。

MeDIP-process

 

《MeDIP 的两种广泛应用》

 

      艾美捷科技作为专业的生命科学领域解决方案供应商,为您推荐来源于全球最畅销,发表文章最多的甲基化DNA免疫沉淀(MeDIP)试剂盒:  

 amyjet tel
产品名称及描述 货号 产品说明
Methylamp Methylated DNA Capture (MeDIP) Kit P-1015 详情
  •       该试剂盒需要的样品:提取好的基因组DNA

 

       MeDIP试剂盒原理: Methylamp甲基化DNA免疫沉淀试剂盒中含有捕获样品中甲基化DNA所需的所有试剂,并且,本试剂盒中还有一种CHIP-级5-MC抗体,以及正常小鼠的IgG阴性对照.DNA打断之后,加入微孔板中,被抗体捕获.然后DNA从复合物上解离下来,通过本公司特别设计的高速离心柱子纯化,洗脱下来的DNA可以被用于随后的各种扩增反应。

 

       MeDIP试剂盒优势:

      1、高效地浓缩富集甲基化DNA,>98%;

      2、同类产品中最快速的试剂盒,3小时内完成反应;

      3、96孔板模式使研究人员能根据自己需要选择手工或是高通量分析;

      4、附有DNA纯化柱子:节省时间和人力;

      5、操作简便、结果可靠、统一的分析条件;

 

      实验流程示意图与结果展示:

 

  MeDIP-result  

 

 

  《已发表28篇文章

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