8-羟基脱氧鸟苷(8-OHdG)定量试剂盒:墙裂推荐直接法

发布者:艾美捷科技发布时间:2018-06-26

8-羟基脱氧鸟苷

 

      活性氧(ROS)自由基可以直接攻击生物大分子DNA诱发DNA氧化损伤。在各种氧化损伤中,鸟苷是DNA核碱基中最易被氧化的碱基,羟基自由基和超氧阴离子会直接攻击DNA分子中鸟嘌呤碱基的第8位碳原子,从而生成氧化性加合物8-OhdG(8-羟基脱氧鸟苷)。  

8-OhdG(8-羟基脱氧鸟苷)

 

      8-OHdG现已被广泛地应用在实验研究和临床诊断中,参与氧化应激与病变的指标检测与机理分析,对心血管疾病、糖尿病、神经退行性疾病(帕金森病,阿尔茨海默氏症)、以及癌症的临床诊疗都具有极大的应用价值。目前市场上主流使用的是竞争ELISA方法,因为它们不够精确,也不便于应用于细胞/组织的8-OHdG检测,更不能直接检测从细胞或组织分离的完整DNA。   为此,艾美捷科技作为专业的生命科学领域解决方案供应商,为您推荐全球最新的8-OHdG直接定量试剂盒。其检测准确度与HPLC或LC-MS分析高度相关和接近。  

 

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产品名称及描述 货号 产品说明
EpiQuik 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric) P-6003 详情

 

*8-OHdG试剂盒可直接检测提取好的DNA样品

 

      8-羟基脱氧鸟苷试剂盒原理

 

      DNA包被特殊处理带有高DNA亲和性的微孔板上,8-OHdG通过捕获抗体和检测抗体来检测.通过酶标仪可以检测结果.大量得8-OHdG与光密度值成比例.  

 

8-OHdG试剂盒实验流程

 

 

<8-OHdG试剂盒实验流程,标准曲线,以及与液相质谱分析对比>

 

 

      8-OHdG试剂盒优势:

 

      1、比色实验简单易用,方便快速,整个试验完成需要3小时45分钟;

      2、高灵敏度,检测8-OHdG极限低至2pg;

      3、高特异性,只能检测8-OHdG,不能检测8-OHdG类似物;

      4、利用完整DNA直接检测8-OHdG,能消除高分子量化合物的影响,例如:糖类和蛋白质等能竞争8-OHdG;

      5、检测结果精确,近似接近于HPLC和LC-MS;

      6、高度方便直接使用来自细胞,组织中的DNA,不需要DNA消化或者水解;

      7、包含通用的阳性对照和阴性对照,能适用于定量任何种系8-OHdG;

      8、本试剂盒实验结果更精确,灵敏,可靠,可靠并能显著降低背景信号.

 

      《8-OHdG试剂盒已发表文章》

 

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